INTERNATIONAL WORKSHOP on the SKELETAL GROWTH PLATE

 

SCIENTIFIC PROGRAM

 

Sunday, June 11

 

4:00pm:                       Check-in Registration Begins

 

5:30 – 7:00pm:           Dinner

 

7:30pm:                       WELCOME and INTRODUCTION TO WORKSHOP AND TO THE GROWTH PLATE

-William Horton, Shriners Hospital/OHSU, Portland

 

7:45 – 9:00 pm:          Session 1: OVERVIEW

 

   7:45 – 8:15               Stefan Mundlos, Max Planck Institute of Medical Genetics, Berlin, Germany – Developmental aspects of skeletal disease.

 

   8:15 – 8:30               Rocky Tuan, NIAMS/NIH, Bethesda – Introduction of cartilage

                                    engineering.

 

   8:30 – 9:00               Cornelia Farnum,  Cornell University School Veterinary Medicine, Ithaca, NY – The dynamics of post-natal bone elongation: observations using in vivo-based approaches.

 

9:00pm:                       Wine & Cheese Social

 

 

Monday, June 12

 

7:30 – 8:30am:           Continental Breakfast

 

7:30 – 9:00am:           Check-in Registration

 

8:30 – 10:00am:         Session 2:  ORIGIN AND DIFFERENTIATION OF GROWTH PLATE CHONDROCYTES

                                    Session Chair: Bjorn Olsen

 

   8:30 – 9:00               Veronique Lefebvre, Cleveland Clinic Foundation, Cleveland – Sox 9, Sox 5 and Sox 6 in chondrocyte differentiation.

 

9:00 – 9:30               T. Michael Underhill, University of British Columbia, Vancouver, Canada – BMP Action in skeletogenesis involves attenuation of retinoid signaling

 

   9:30 – 9:45               Karen Lyons, UCLA, Los Angeles – Roles of BMPR1A and BMPR1B in early chondrogenesis.

 

   9:45 – 10:00             Christine Hartmann, Research Institute of Molecular Pathology, Vienna, Austria – A Wnt canon orchestrating skeletogenesis.

 

10:00 – 10:30am:       Break

 

10:30 – 12:30pm:       Session 3:  PROLIFERATION OF GROWTH PLATE CHONDROCYTES

                                    Session Chair - Phyllis LuValle

 

   10:30 – 11:00           Phyllis LuValle, University of Florida, Gainsville – Activation of cyclin D1 in proliferating chondrocytes.

 

   11:00 - 11:30           Jeffrey Baron, NICHD/NIH, Bethesda – The role of the resting zone in growth plate chondrogenesis.

 

   11:30 – 12:00           Peter Hurlin, Shriners Hospital/OHSU, Portland – N-Myc and c-Myc regulation by FGF receptor signaling.

 

   12:00 – 12:15           Razvan Miclea,  Leiden University, Leiden, Netherlands - APC-mediated β-catenin degradation is essential for chondrocyte formation from mesenchymal precursors.

 

   12:15 – 12:30           Elazar Zelzer,  Weizmann Institute, Rehovot, Israel - VEGF coordinates the coupling of chondrogenesis and angiogenesis in the developing limb.

 

12:30 – 1:30pm:         Lunch

 

1:45 – 2:15 pm:          David Rimoin, UCLA/Cedars Sinai, Los Angeles – Skeletal Dysplasia Nosology

 

2:15 – 4:00pm:           Session 4:  POST-MITOTIC EVENTS IN THE “PROLIFERATIVE ZONE”

                                    Session Chair - Reinhard Fδssler

 

    2:15 – 2:45              Reinhard Fδssler, Max Planck Institute, Martinsried, Germany – β1 integrin mediates chondrocyte rotation in the growth plate.

 

    2:45 – 3:15              Bjorn Olsen, Cell Biology, Harvard – Growth plate chondrocyte polarity

 

    3:15 – 3:45              Silvio Garofalo, University of Molise Medical School, Naples, Italy – Misexpression of hedgehog disturbs growth plate cell orientation.

 

   3:45 – 4:15               Benoit St. Jacques, Shriners Hosptial/McGill University, Montreal – Transcriptional regulation of Ihh by FGF signaling in growth plate chondrocytes.

 

   4:15 – 4:45               Maurizio Pacifici, Jefferson Medical College, Philadelphia -

                                 Growth plate organization, function and defects in Ihh- and

                                 Ihh/Kif3a-null mice.

 

5:30 – 6:30pm:           Dinner

 

7:00 – 9:30pm:           Session 5:  HYPERTROPHY OF GROWTH PLATE

                                 CHONDROCYTES

                                 Session Chair -  Henry Kronenberg

 

   7:00 – 7:30               Henry Kronenberg, Endocrine Unit, MGH/Harvard – PTHrP control of terminal differentiation.

 

   7:30 – 8:00               Brendan Lee, Baylor College of Medicine, Houston – Transcriptional regulation of terminal chondrocyte differentiation.

 

   8:00 – 8:15               Sherrill Adams, University of Pennsylvania, Philadelphia - Chick collagen X promoter activity is stimulated by a T3-retinoid-BMP cascade.

 

   8:15 – 8:45               Regis O’Keefe, University of Rochester, Rochester – Smads mediate chondrocyte hypertrophy and bone growth.

 

8:45 – 9:15               John Bateman, Murdoch Research Institute, Melbourne, Australia – Loss of type X collagen in Schmid metaphyseal chondrodysplasia.

 

   9:15 – 9:30               Pia Hermanns, Center of Youth & Adolescence Medicine, Freiburg, Germany - The effect of RMRP mutations in cartilage hair hypoplasia.

 

 

Tuesday, June 13

 

7:30 – 8:30am             Continental Breakfast

 

8:30 – 10:00am           Session 6:  MINERALIZATION OF THE GROWTH PLATE

                                 Session Chair - Francis Glorieux

 

   8:30 – 9:00               Francis Glorieux, Shriners Hospital/McGill University, Montreal – Mutations of vitamin D (3) 1α hydroxylase in men and mice.

 

   9:00 – 9:30               Barbara Boyan, Georgia Tech, Atlanta – Hormonal regulation of chondrocyte maturation and mineralization.

 

9:30 – 10:00             H. Clarke Anderson, University of Kansas Medical School, Kansas City - Mineralization of matrix vesicles in rachitic cartilage.

 

   10:00 – 10:30           Rene St. Arnaud, Shriners Hospital/McGill University, Montreal - Disturbed bone growth and mineralization in mouse model of

                                 pseudovitamin D-dependent rickets.

 

10:30 – 11:00am:       Break

 

   11:00 – 11:30           Thorsten Kirsch,  University of Maryland Medical School, Baltimore – Annexin and growth plate maturation and mineralization.

 

   11:30 – 12:00           A. Hari Reddi,  University of California, Davis – BMPs in cartilage engineering.

 

   12:00 – 12:15           Klaus von der Mark,  University of Erlangen, Erlangen, Germany - Collagen X- BAC constructs control specific and efficient LacZ expression in hypertrophic chondrocytes in transgenic reporter mouse lines.

 

   12:15 – 12:30           Christa Maes, Endocrine Unit, MGH, Harvard - Characterization of the osteoblast lineage in vivo during early bone development.

 

   12:30 – 12:45           Malgorzata Wiweger, The University of Sheffield, UK – HSPGs in zebrafish cartilage and bone development.

 

12:45 – 1:45pm:         Lunch

 

4:00 – 5:30pm:           Poster Session

 

5:30 – 6:30pm:           Dinner

 

7:00 – 9:45pm:           Session 7:  TERMINAL EVENTS IN THE GROWTH

                                    PLATE:  REMODELING, VASCULAR INVASION AND

                                    MARROW ESTABLISHMENT.

                                    Session Chair - Zena Werb

 

   7:00 – 7:30               Zena Werb,  UCSF, San Francisco – Vascular invasion and matrix remodeling in the growth plate. 

 

7:30 – 8:00               Ernestina Schipani, Endocrine Unit, MGH/Harvard – Role of Hif-1alpha in differentiation of limb bud mesenchyme and joint development.

 

8:00 – 8:30               Olena Jacenko,  University of Pennsylvania, Philadelphia – Linking chondrocyte hypertrophy and endochondral ossification to

                                    hematopoiesis through analysis of transgenic mouse models.

 

   8:30 – 9:00               Scott Saunders,  Washington University, St. Louis – Delayed endochondral ossification and altered hematopoiesis in

                                    glypican-3 null mice.

 

9:00 – 9:30                Danny Chan,  Hong Kong University, Hong Kong – Mouse model with impaired collagen X degradation at the chondro-osseus junction.

 

 

 

Wednesday, June 14

 

7:30 – 8:30am:           Continental Breakfast

 

8:30 – 10:00am:         Session 8:  EXTRACELLULAR REGULATION OF

                                    CHONDROCYTE PROLIFERATION

                                    Session Chair - David Ornitz

 

    8:30 – 9:00               David Ornitz,  Washington University, St. Louis – FGFR3 regulation of growth plate chondrocyte proliferation.

 

  9:00 – 9:30                Andrea Vortkamp,  University of Essen, Essen, Germany – Ihh signaling in distal chondrocytes.

 

  9:30 – 10:00              John Hassell, Shriners Hospital/University of South Florida, Tampa – Cartilage proteoglycans influence growth factor transport and cellular responses in the growth plate.

 

   10:00 – 10:15            Kathryn Rodgers PhD:  University of Pennsylvania, Philadelphia – A Hypomorphic Perlecan Mouse Model for Schwartz-Jampel syndrome.

 

10:15 – 10:45am:       Break

 

   10:45 – 11:15                       Lynn Sakai, Shriners Hospital/OHSU, Portland– Fibrillins

                                    bind BMPs and influence growth plate chondrogenesis. 

 

   11:15 – 11:45                       Linda Sandell, Washington University, St Louis – Type II

                                    collagen propeptides bind growth factors in cartilage matrix.

 

   11:45 – 12:15           Benjamin Alman,  University of Toronto, Toronto – Dysregulation of growth plate regulatory circuits in skeletal tumors.

 

12:15 – 1:30pm:         Lunch

 

4:00 – 5:00pm:           Poster Session

 

5:00 – 7:45pm:           Session 9:  DISTURBANCES OF CHONDROGENESIS

                                    Session Chair – Olena Jacenko

 

   5:00 – 5:30               Matthew Warman,  Case Western Reserve, Cleveland – Mutations of C-type natriuretic hormone receptor in human acromesomelic dysplasia.

 

   5:30 – 5:45               William Wilcox,  UCLA/Cedars-Sinai, Los Angeles - C-type natriuretic peptide (CNP) blocks prolonged Erk activation by FGF-receptor 3 (FGFR3) that causes chondrocyte proliferation arrest and extracellular matrix degradation.

 

   5:45 – 6:15               William Horton, Shriners Hospital/OHSU, Portland –

                                    Modulation of FGFR3 signals in achondroplasia.

 

   6:15 – 6:45               Avner Yayon,  ProChon Biotech Ltd, Rehovot, Israel – Strategies to block FGFR3 signals in mouse models of achondroplasia.

 

   6:45 – 7:15               Hans Peter Bδchinger, Shriners Hospital/OHSU, Portland - Disturbances of matrix protein biosynthesis and secretion.

 

   7:15 – 7:45               Kathryn Cheah,  Hong Kong University, Hong Kong – ER stress in the hypertrophic chondrocyte.

 

   7:45 – 8:00               Paul Holden, Shriners Hospital/OHSU, Portland – Cartilage oligomeric matrix protein: disease mutations and mechanisms.

 

8:10 – 9:50pm:           Banquet Dinner

 

 

Thursday, June 15

 

7:30 – 8:30am:           Continental Breakfast

 

8:30 – 11:45am:         Session 10:  RECAPITULATION OF THE GROWTH PLATE

                                    CHONDROCYTE LIFE CYCLE IN CARTILAGE

                                    ENGINEERING.

                                    Session Chair – Rocky Tuan

 

    8:30 – 9:00              Brian Johnstone,  Oregon Health & Science University, Portland – Application of mesenchymal stem cells for skeletal tissue engineering.

 

    9:00 – 9:30              Rocky Tuan,  NIAMS/NIH, Bethesda – Mesenchymal stem cells and scaffold development in cartilage engineering.

 

9:30 – 10:00             Robert Guldberg,  Georgia Tech, Atlanta – Micro CT – imaging of the growing skeleton.

 

   10:00 – 10:15           Juan Taboas,  NIAMS/NIH, Bethesda - Cross-talk between chondrocyte and endothelial cells

 

   10:15 – 10:30           Rebecca Williams,  Cornell University, Ithaca - In vivo visualization of tracer transport using multiphoton microscopy.

                       

10:30:                          Rocky Tuan PhD:  NIAMS/NIH, Bethesda – Wrap-up discussion.